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Two-step RT real-time-PCR approach In this approach, the mRNA was reverse transcribed with the MMLV reverse transcriptase into cDNA separately on another platform (TGradient, Biometra, G¨ottingen, Germany). The studied flavanols were added after the RT reaction. The two-step RT real-time PCR was employed additionally to study the effect of flavanol exclusively on the polymerase reaction

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The presence of Azotobacter sp. in soils has beneficial effects on plants, however abundance of these bacteria is related to many factors, especially to soil pH and fertility.

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