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Neural circuitry of a polycystin-mediated hydrodynamic

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To remove residual template DNA, 2 units of TURBO DNase (Ambion) were added and incubated at 37 C for 60 minutes. The M. tuberculosis tRNAfMET was then purified using the MEGAclear kit (Ambion), following the manufacturer’s protocol. M. tuberculosis VapC30 protein was mixed with tRNAfMet and incubated at 37 C for 60 min. Digested RNA fragments were analyzed by denaturing 15% acrylamide …

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Modified mRNA was prepared with a T7 polymerase-based IVT kit (Ambion, Austin, TX) according to the manufacturer's manual. The open-reading frame of the gene of interest was cloned into a pcDNA3.1 vector under the regulation of T7 promoter. The untranslated region and gene of interest was amplified by PCR reaction with 5′ primer CTAGAGAACCCACTGCTTACTGGCTTATCG and 3′ primer T



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After transcription, the transcribed product was purified using MEGAclear® columns (Ambion, USA). [0090] Synthesizing the First Strand of Complementary DNA [0091] To 25 ng of synthetic messenger RNA there was added 1.25 μl of N6-T7 composite primers (Thermo Electron, Germany) and the tube was incubated for 10 min at 70° C. followed by 10 min on ice (4° C.).

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Neural circuitry of a polycystin-mediated hydrodynamic

1 Washington University in St. Louis Washington University Open Scholarship All Theses and Dissertations (ETDs) Spring Characterization of Human Gut Microbiota Dynamics Using Model Communities in Gnotobiotic Mice Nathaniel Patrick McNulty Washington University in St. Louis Follow this and additional works at: Part of the Microbiology Commons

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The present disclosure describes the C-type lectin CLEC1 la as a bone growth factor. C1ec11a-deficient mice showed reduced bone volume and mineralization, while bone resorption remained unchanged. Administration of recombinant Clecl la systemically promoted bone formation in …

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Patent application title: Replication-proficient dsRNA capsids and uses thereof Inventors: David Hone (Poolesville, MD, US) David Onyabe (Poolesville, MD, US)

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Neural circuitry of a polycystin-mediated hydrodynamic

To remove residual template DNA, 2 units of TURBO DNase (Ambion) were added and incubated at 37 C for 60 minutes. The M. tuberculosis tRNAfMET was then purified using the MEGAclear kit (Ambion), following the manufacturer’s protocol. M. tuberculosis VapC30 protein was mixed with tRNAfMet and incubated at 37 C for 60 min. Digested RNA fragments were analyzed by denaturing 15% acrylamide …

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Patent application title: Replication-proficient dsRNA capsids and uses thereof Inventors: David Hone (Poolesville, MD, US) David Onyabe (Poolesville, MD, US)

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Efficient inversions and duplications of mammalian

9/01/2018 · Transmission Electron Microscopy The proteins for TEM studies were expressed in E. coli and purified as described above. Samples containing either SOD1 (0.2 mM) alone or SOD1 with equimolar concentrations of selected nanobodies were imaged after incubation for 4 weeks with shaking in 50 mM Tris-HCl (pH 8) at 25° C.

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